研究テーマ / Research Interest

水中の健康関連微生物(特に腸管系ウイルス)

Health Related Water Microbiology (Enteric Virus, Especially)


【これまでの論文で取り扱ったテーマ群 / Research Themes in My Previous Studies】

  ・水中ウイルスの検出阻害(PCR阻害)  Inhibitory effects on PCR-based virus detection from water

          ・阻害の評価方法   Evaluating the effect of the inhibition

          ・阻害物質の特性評価 Characterizing the inhibitory substances

          ・阻害の軽減方法   Mitigating the inhibitory effect

  ・水中ウイルスの濃縮法   Concentration methods for viruses in water

  ・水中ウイルスの検出法   Detection methods for viruses in water

          ・FRNAファージの定量ジェノタイピング   Quantitative genogrouping of FRNA phages

          ・アイチウイルスのPCR系   Quantitative PCR assay for Aichi virus 1

          ・アストロウイルス(MLB,VA含む) のPCR系   PCR assays for human astroviruses (classic and MLB- and VA-clades)

          ・東京湾,琵琶湖,桂川,石巻湾   Tokyo-Bay, Lake Biwa, Katsura River, Ishinomaki-Bay

          ・タイ,カンボジア   Thailand, Cambodia

  ・水処理によるウイルス減衰評価   Evaluation of virus reduction by water treatments

          ・活性汚泥法   activated sludge

          ・凝集沈殿   coagulation-sedimentation

          ・砂ろ過法   sand filtration

          ・限外ろ過   ultrafiltration

          ・紫外線   UV

 

【これまでの論文で取り扱った微生物群 / Microbes targeted in our previous studies】

          ・ノロウイルス   noroviruses

          ・サポウイルス   sapovirus

          ・アストロウイルス   astroviruses (including MLB- and VA-clades)

          ・ロタウイルス   rotavirus

          ・エンテロウイルス(ポリオウイルス含)   enteroviruses

          ・アイチウイルス   Aichi virus 1

          ・トウガラシ微斑ウイルス   pepper mild mottle virus

          ・Fファージ   F-specific bacteriophages (FRNA phages, especially)

          ・アデノウイルス adenoviruses

          ・ポリオーマウイルス   polyomaviruses

          ・大腸菌,大腸菌群   E.coli, total coliforms

          ・SARS コロナウイルス-2   SARS coronavirus-2

学術論文(査読有)/ Peer reviewed paper


Hata A, Katayama H, Wattanachira S, Sethy S, Masago Y, Honda R, Matsui Y. Removal of microbes from highly turbid surface water in Southeast Asia using ceramic membrane filters, Southeast Asian Water Environment, IWA Publishing, 3. pp. 153-158, 2010.

 

Hata A, Katayama H, Kitajima M, Visvanathan C, Nol C, Furumai H. Validation of Internal Controls for Nucleic Acid Extraction and Amplification of Enteric Viruses in Water Samples, Applied and Environmental Microbiology, American Society for Microbiology. 7713, pp. 4336-4343, 2011.

 

端昭彦,稲葉愛美,片山浩之,古米弘明,震災後の石巻沿岸域における腸管系ウイルス及び指標微生物の検出と検出阻害評価,土木学会論文集G (環境),公益社団法人土木学会,第68巻,第7号,pp. III_285-III-2962012年.

 

Hata A , Kitajima M, Katayama H. Occurrence and reduction of human viruses, F-specific RNA coliphage genogroups and microbial indicators at a full-scale wastewater treatment plant in Japan, Journal of Applied Microbiology, Wiley, 1142, pp. 545-554, 2013.

 

稲葉愛美,片山浩之,Tran Thi Viet Nga端昭彦,古米弘明,環境水中の糞便汚染指標としてのブタコブウイルスおよびウシコブウイルス,用水と廃水,産業用水調査会,第55巻,第5号,pp.371-3772013年.

 

Kitajima M, Hata A, Yamashita T, Haramoto E, Minagawa H, Katayama H. Development of a Reverse Transcription-Quantitative PCR System for Detection and Genotyping of Aichi Viruses in Clinical and Environmental Samples, Applied and Environmental Microbiology, American Society for Microbiology. American Society for Microbiology, 7913, pp. 3952-3958, 2013.

 

Hata A, Katayama H, Kojima K, Sano S, Kasuga I, Kitajima M, Furumai H. Effects of rainfall events on the occurrence and detection efficiency of viruses in river water impacted by combined sewer overflows, Science of the Total Environment. Elsevier, 468-469C, pp. 757-763, 2014.

 

Hata A, Kitajima M, Tajiri-Utagawa E, Katayama H. Development of a High Resolution Melting Analysis for Detection and Differentiation of Human Astroviruses, Journal of Virological Methods. Elsevier, 200C, pp. 29-34, 2014.

 

Hata A, Matsumori K, Kitajima M, Katayama H. Concentration of Enteric Viruses in Large Volumes of Water Using a Cartridge-Type Mixed Cellulose Ester Membrane, Food and Environmental Virology, Springer, 7,1, pp. 7-13, 2015.

 

Hata A, Katayama H, Furumai H. Organic Substances Interfere with Reverse Transcription-Quantitative PCR-Based Virus Detection in Water Samples, Applied and Environmental Microbiology. American Society for Microbiology, 81巻,第5号,pp. 1585-15932015.

 

Hata A, Katayama H, Kitajima M, Furumai H. Wastewater Analysis Indicates that Genetically Diverse Astroviruses, Including Strains Belonging to Novel Clades MLB and VA, are Circulating Within Japanese Populations, Applied and Environmental Microbiology. American Society for Microbiology, 81巻,第15号,pp. 4932-49392015.

 

Sano D, Amarasiri M, Hata A, Watanabe T, and Katayama H. Risk management of viral infectious diseases in wastewater reclamation and reuse: Review, Environmental International. Elsevier, 91, pp. 220-229, 2016.

 

Hata A, Hanamoto S, Shirasaka Y, Yamashita N, and Tanaka H. Quantitative distribution of infectious F-specific RNA phage genotypes in surface waters, Applied and Environmental Microbiology. American Society for Microbiology,8214, pp. 4244-4252, 2016.

 

Lee S, Hata A, Yamashita N, and Tanaka H. Evaluation of virus reduction by ultrafiltration with coagulation–sedimentation in water reclamation, Food and Environmental Virology. Springer, 94, pp. 453-463, 2017.

 

Hata A, Inaba M, Katayama H, and Furumai H. Characterization of Natural Organic Substances Potentially Hindering RT-PCR-Based Virus Detection in Large Volumes of Environmental Water, Environmental Science and Technology. ACS Publications, 5123, pp. 13568-13579, 2017.

 

Lee S, Tasaki S, Hata A, Yamashita N, and Tanaka H. Evaluation of virus reduction at a large-scale wastewater reclamation plant by detection of indigenous F-specific RNA bacteriophage genotypes, Environmental Technology. Taylor and Francis, 第40巻,第19号,pp.2527−2537, 2018.

 

Haramoto E, Kitajima M, Hata A, Torrey JR, Masago Y, Sano D, and Katayama H. A review on recent progress in the detection methods and prevalence of human enteric viruses in water, Water Research. Elsevier, 135, pp. 168-186, 2018.

 

Hata A, Haramoto E, Kitajima M, Lee S, Ihara M, Gerba CP, and Tanaka H. Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters, Scientific Reports. Nature, 第8巻,第1号,11837, 2018.

 

Hata A, Hanamoto S, Ihara M, Shirasaka Y, Yamashita N, Tanaka H. Comprehensive Study on Enteric Viruses and Indicators in Surface Water in Kyoto, Japan, During 2014-2015 Season, Food and Environmental Virology. Springer, 第10巻4号, pp. 353-364, 2018年.  

 

Liu M, Hata A , Katayama H, Kasuga I. Consecutive ultrafiltration and silica adsorption for recovery of extracellular antibiotic resistance genes from an urban river. Environmental Pollution. Elsevier, 第260巻, 114062. 2020年.  

 

Hata A, Furumai H, Katayama H. Sequential treatment using a hydrophobic resin and gel filtration to improve viral gene quantification from highly complex environmental concentrates. Water Research. Elsevier, 第174巻, 115652. 2020年.

 

Hata A, Hara-Yamamura H. Meuchi Y. Imai S. Honda. R. Detection of SARS-CoV-2 in wastewater in Japan during a COVID-19 outbreak. Science of the Total Environment. Elsevier, 第758巻, 143578. 2021年.

 

Kumar M. Md. Alamin, Kuroda K. Dhangar K. Hata A. Yamaguchi H. Honda R. Potential discharge, attenuation and exposure risk of SARS-CoV-2 in natural water bodies receiving treated wastewater. npj Clean Water. Nature, 第4巻, 8. 2021年.

 

Hata A. Shirasaka Y. Ihara M. Yamashita N. Tanaka H. Spatial and temporal distributions of enteric viruses and indicators in a lake receiving municipal wastewater treatment plant discharge. Science and Total Environment. Elsevier, 第780巻, 146607. 2021年.

解説・報告等


Murakami M, Hata A, Honda R, Watanabe T. Letter to the Editor: Wastewater-Based Epidemiology Can Overcome Representativeness and Stigma Issues Related to COVID-19. Environmental Science and Technology. ACS Publications, 54(9):5311. 2020年

 

Hata A, Honda R. Potential Sensitivity of Wastewater Monitoring for SARS-CoV-2: Comparison with Norovirus Cases. Environmental Science and Technology. ACS Publications, 54(11):6451-6452.  2020年

 

端昭彦.とやまの土木ー過去・現在・未来(33)感染症疫学情報集積の場としての下水処理場.実業之富山web版.  2020年

 

井口晃徳,端昭彦. WET2020-online開催報告.水環境学会誌.44(3): 92-95. 2021年

 

Hata A, Honda R, Hara-Yamamura H, Meuchi Y. Identification of SARS-CoV-2 in wastewater in Japan by multiple molecular assays-implication for wastewater-based epidemiology (WBE), medRxiv. https://doi.org/10.1101/2020.06.09.20126417. 2020年

 

池端慶裕,浅田安廣,籠田大介,端昭彦,伊藤万葉,村田道拓,長尾麻未.IWA世界会議・展示会を振り返る 〜寄稿・若手専門家が見た「世界」〜.水道公論.54(11).33-47. 2018年

 

水中の健康関連微生物研究委員会(片山浩之,安井宣仁,猪又明子,田中宏明,岸田直裕,原本英司,渡部徹,真砂佳史,浅田安廣,端昭彦,佐野大輔,井原賢,稲葉愛美).水中の健康関連微生物に関する研究動向.水環境学会誌,公益社団法人日本水環境学会,第37巻,第12号,pp. 424−433,2014年.

 

端昭彦.ウイルス濃縮過程における共存有機物の濃縮とウイルス検出阻害.水環境学会誌.35(12): 412.(オルガノ賞受賞報告).2012年

 

端昭彦.【近況報告】学会報告〜IWAの水環境健康関連微生物シンポジウム@ニュージーランド〜.Japan National Young Water Professionals Newsletter.第3号,pp.3. 2011年